English Guide: Practical Tips for Better English Communication

Improve your English vocabulary by learning root words

A rich vocabulary is an important indicator of a person’s command over the English language. That is one of the reasons why we keep offering tips regularly on how to improve your English vocabulary. However, building one’s vocabulary requires sustained effort to discover and add new words that can be used effectively in your communication. The process of enriching one’s vocabulary can be simplified into 4 key steps: read a lot of good English books,...

Essential skills for the workplace in the 2020s

The last two decades have been hugely transformational as digital technologies have disrupted every walk of life. This will only continue in the new decade, as machine learning, deep learning and artificial intelligence (AI) begin to play an integral part in our workplaces. As a result, workplaces will require very different skillsets than they needed until now. If you are a youngster looking for rapid career development in this new decade, then...

How to score band 7 and above in IELTS?

If you are planning to study, work or even immigrate to any western country (Australia, New Zealand, US, Canada, UK – to name a few), you will most likely be required to get a good IELTS score to get the requisite entry visa. Typically, this means that you should get a score of band 7 or higher to maximise the chances of getting the visa approval. The IELTS score contributes significant points to the points-system used by many countries for...

Voracious reading: The real secret to building a strong English Vocabulary

Recently, the former Union Minister and former top UN diplomat Mr.Shashi Tharoor was asked the secret of his exceptional English vocabulary. While he joked initially, he then gave the secret of his amazing and enviable vocabulary: Reading. Yes, the open secret to a strong and deep English vocabulary is as simple as that- a lot of reading of good English books. Mr.Tharoor dispelled the notion that he moves around carrying an English dictionary....

5 English phrases to avoid in conversation and display greater social awareness

Emotional intelligence (used interchangeably with emotional quotient) and social awareness are increasingly important attributes that have a bearing on how one is perceived in a social or professional environment. The words and phrases we use are manifestations of our social awareness and therefore, require careful thought. Following are 5 English phrases that people avoid for their likelihood of being misinterpreted. We list below five of...

Communicate your passion at work for career success

You can find your dream job on Jooble If you are like most people, you would like to achieve professional growth and success in your career. Typically, career success would mean promotions, greater responsibilities and accompanying financial rewards. If you look around you, you will often find that the people who progress and move up the ladder are those who demonstrate passion at work, are proactive, and of course, perform on the job at hand...

How to lodge a telephonic complaint against a faulty bill

It is never a pleasant task to call up the customer service of one of your service providers and complain about any issue you may have encountered, whether it is a faulty bill or inadequate service you have received. Nevertheless, making unpleasant telephone conversations to lodge a complaint is a part of life that one must be prepared for. How you go about making this conversation can go a long way in determining whether you get the desired...

To speak good English, surround yourself by people who speak English fluently

Let us say that your current English speaking skills aren’t great. You aren’t very confident of being able to communicate confidently and effectively in English with anyone, be it a small group or a large one. However, you are very determined to improve your spoken English skills rapidly and prove to yourself that you can do it, while making a good impression on those around you. Taking up an English speaking course is obviously one way to...

How to score “high-band” in IELTS speaking test

If you are planning to take the IELTS test, you would be aware that the test comprises of four different modules: Listening, Reading, Writing and Speaking. For many IELTS test takers, the speaking test is often the most difficult. This is not very surprising because it is akin to speaking in public. It is a known fact that ‘public speaking’ is one of the scariest tasks for people. There are three sections in the speaking test in IELTS. The...

How to improve your spoken English fluency

There is a quote attributed to the writer Stephen King that says that “if you want to be a writer, there are two things you must do: Read a lot and write a lot.” You can extend that quote to say the following: “To be a fluent English speaker, you must listen a lot and speak a lot.” While there is no substitute to this mantra, we will give you some more details of how you could work towards becoming a more fluent speaker. To start with, let’s...

How to communicate with energy for more effective meetings

It is well known that the quality of communication between two or more individuals, impacts the outcome of business meetings. While the exact content of the meeting and its structure are critical, certain external factors like the energy and enthusiasm of the participants in a meeting, also play a very important role in the success or failure of a meeting. In this article, we will offer some simple tips on how you can improve the chances of a...

Why success in a sales career needs great communication skills

A career in sales can be very exciting and lucrative for people who do well in it. Sales brings in revenues for any organisation, and revenues help companies move forward. As long as one is performing well and continues to bring in sales, one’s job is fairly secure because in times of adversity businesses slash costs, while trying to keep revenues high. Yet, success in a sales career isn’t guaranteed for everyone. It takes certain personality...

Why you should focus more on correct English pronunciation, not accent

When it comes to learning spoken English, many people often mistake the purpose of the learning. They tend to think that acquiring English speaking skills is about speaking with a British or American accent. They couldn’t be farther from truth. The true purpose should be to learn to speak English correctly, fluently, confidently and in manner that the listener understands the communication without much difficulty. Too much emphasis is laid on...

Improve your English: know the difference between “affect” and “effect”

In a recent article, we wrote about the difference between improve and improvise and how to use these two similar terms appropriately. Continuing this series on using similar-sounding English words correctly, let us consider the use of the words “affect” and “effect”. Both terms mean “impact” or “result” of something, but their usage varies depending on the context in which they are being used. Affect The word “affect” is generally used in the...

Understand the difference between improve and improvise

English language has plenty of words that seem very similar but mean very different. It is quite easy to ignore the subtle difference in the words themselves and consider them to mean the same thing. This results in incorrect usage of the words when writing or speaking in English, which may confuse the listener or create a negative impression about your English Communication skills. Let’s focus on two words, improve and improvise; the former is a...

How to convince your manager to give you added work responsibilities

Someone has rightly said, “In the mundane, nothing is sacred. In sacredness, nothing is mundane.” Mundane is boring. There comes a time when you feel saturated with the routine tasks you are doing and you feel like doing different and challenging things. Your ardent desire to take up additional work and prove your worth overwhelms you. But this needs to be communicated effectively to your boss. Your boss should be aware that you are geared up...

How to give a thank you speech at your promotion party

Gratitude is of vital importance in human life. We need to thank people who have, directly or indirectly, explicitly or implicitly, made a positive change in our life. ‘Thank You’ is the least that we can say. So never shy away from that. In professional life too, gratefulness is of utmost importance, especially when someone has helped you climb up the career ladder. It’s your promotion party and you have to give a thank you speech – this...

How to ask for a leave extension from your manager

We all have encountered situations wherein there were unavoidable or unforeseen circumstances because of which we had to extend our leave. For example, sickness, family issues, marriage etc. Now here comes the uncomfortable question – how to ask the boss for a leave extension. Quite tricky, huh? When you are already on leave and then you have to request for extension of leave, it should be done in such a way that it is granted without much...

Why speaking good English at the workplace still makes a difference to your career

A few months back, a video of a job prospect venting out his frustrations on having to speak in English at job interviews, went viral. The protagonist of the video, a youngster, who could speak English fluently was angry that he was always asked to speak in English, and he let out his frustration with a series of expletives in Hindi. The fact that the video went viral and was shared by a large audience in the country, especially among...

How to make the most of networking events for career growth

Man is a social animal. We cannot survive in isolation. Also, we all crave for love and attention, no denying that. We are constantly expanding our social network. Be it personal or professional life, networking is of utmost importance to us: Social networking, for getting love and affection, and professional networking for career enhancement. In this competitive world, we cannot lounge around waiting for opportunities to knock at our door. We...

How to improve English Listening and Comprehension Skills

Someone has rightly said, “Good conversation starts with good listening”. Our teachers have often told us that we wouldn’t learn anything new from talking but we will definitely learn something new from listening. How true! There’s a lot of buzz about spoken English these days, but people tend to forget that English listening is also an integral and inseparable part of English communication, which cannot be overlooked.No wonder then the...

Why good English is important for a career in SEO

India is undergoing a digital revolution. The country has embarked on the ‘Digital India’ movement that has been officially promoted by the Indian government. It is therefore not surprising to see a jump in career opportunities in the field of digital marketing, especially search engine marketing (SEM). This field attracts a lot of freshers and young job aspirants. Having good English skills will enable a person to take advantage of the growing...

Restaurant Etiquette – How to create afavourable impression when eating out

Good manners will open doors that the best education cannot.” Etiquette is the science of living. It embraces everything.People all over the world are judged, liked, disliked, remembered or looked down with contempt based on their manners. Good manners speak a lot about your personality. It is imperative that you display your best behaviour when you are in a public place especially when eating out. When you walk into a restaurant you don’t want to...

How to compliment your office colleagues in English

Good teamwork is essential not only for the growth of any organisation but also for the personal growth of individuals in the organisation. An essential component of achieving good team bonding is when team members express positive thoughts about fellow team members to them directly. Sincere compliments and praise go a long way in making a person feel good and feel motivated to perform better for the team and the organisation. There are a...

How to request for compassionate leave in your office

At the outset, let us state that we hope you never have to apply for a compassionate leave. However, the reality is that most of us, at some stage of our life, end up having to request for such leaves. This article is written to specifically help you in case of such an eventuality. Before we get into the mechanics of applying for compassionate leave, let us first understand what is compassionate leave. It is leave granted to an employee upon death...

DNA Modifying Enzymes

Created: Tuesday, 19 July 2011 06:21 | Published: Tuesday, 19 July 2011 06:21 | Written by Super User | Print | Email

dna_enzymes-1 You know how to cut a paper or ribbon and paste it again with glue. Similarly, enzymes help to cut and paste DNA fragments. By this way the sequences of DNA would be modified by the enzymes. Each enzyme has its own specific function; let’s discuss one by one.

Enzymes reacts on DNA

Nucleases
Ligases
Topo isomerases
Helicases
Polymerases

Nucleases

By catalyzing the hydrolysis of the phosphodiester bonds, these enzymes cut DNA helixes.

2 types:

1. Exonucleases - hydrolyse nucleotides from the ends of DNA strands
2. Endonucleases cut within DNA strands.

The restriction endonucleases cut DNA strands at specific sequences. To cut the DNA, a restriction enzyme makes two incisions. For example EcoRI digestion happens in the specific base sequence leaving the strand as sticky ends.

Restriction Modifications System:

Restriction enzymes protect bacteria against the bacteriophage virus infection by digesting the phage DNA which penetrates the cell. It is also used in molecular cloning and DNA fingerprinting.

Ligases:

The enzymes which rejoin the broken DNA strands are DNA ligases. Ligases are very crucial in lagging strand DNA replication. Because, they join the short DNA segments and make a complete copy of DNA Template. It is very much essential for genetic recombination and DNA repair.

Topoisomerases:

It has both nuclease and ligase activity. The amount of supercoiling in DNA can be changed by these enzymes. Some of these enzymes cut the DNA strand and rotate one section and reduce the super coiling level. Then the DNA break would be sealed by these enzymes. It is very much essential for DNA Transcription and DNA Replication.

Helicases:

These enzymes use the chemical energy ATP to break hydrogen bonds between bases. So the DNA double helix unwinds and forms two single strands.

Polymerases

It synthesizes polynucleotide chains from nucleoside triphosphates. Polymerases add nucleotides onto the 3′ hydroxyl group of the previous nucleotide in a DNA strand. So, all polymerases work in a 5′ to 3′ direction.

DNA Replication:

DNA polymerase makes a copy of a DNA sequence by a proofreading activity. Hence the accuracy is confirmed. RNA-dependent DNA polymerases copy the sequence of an RNA strand into DNA. dna_enzymes-6

The enzyme reverse transcriptase involved in the infection of cells by retroviruses.

The enzyme telomerase is required for the replication of telomeres. Telomerase contains its own RNA template as part of its structure.

DNA Transcription

Transcription is controlled by RNA polymerase. This enzyme copies the DNA sequence into RNA. It binds to a promoter and separates the DNA strands. It then copies the gene sequence into a mRNA transcript until reaches the terminator and halts and detaches from the DNA segment.

RNA polymerase II transcribes the genes of human genome.

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